Assisted hatching in mouse embryos using a noncontact Ho:YSGG laser system
نویسندگان
چکیده
منابع مشابه
investigating the effect of laser assisted hatching on the development and quality of vitrified-warmed 4-cell stage mouse embryos
objective: the aim of this study was to investigate the effect of laser assisted hatching on the development and quality of vitrified-warmed 4-cell stage mouse embryos. materials and methods: the vitrified-warmed 4-cell mouse embryos were divided into two groups: control group (without laser assisted hatching) and experiment group (with laser assisted hatching). all embryos in both groups were ...
متن کاملThe Use of Laser-assisted Hatching in Bovine in Vitro Produced Embryos to Improve Pregnancy Rate
The Use of Laser-Assisted Hatching in Bovine in Vitro Produced Embryos to Improve Pregnancy Rate. (August 2008) Suzanne Lynn Menges, B.S., Texas A&M University Chair of Advisory Committee: Dr. Duane Kraemer In vitro produced (IVP) embryos not hatching from the zona pellucida (ZP) after transfer is one possible contributing factor of a lower pregnancy rate when compared to in vivo embryos. This ...
متن کاملLaser-assisted hatching of embryos in women of advanced age after in vitro fertilization: a preliminary report.
BACKGROUND For infertile women aged over 37 years, failure of the zona pellucida to rupture is believed to be associated with a decreased implantation rate in in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). It has been suggested that assisted hatching of embryos will increase the pregnancy rate with IVF by means of mechanically facilitating the hatching process. METHO...
متن کاملEffects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers
BACKGROUND It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers. METHODS In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the z...
متن کاملEffects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development
Objective This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers. Methods First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinni...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 1995
ISSN: 1058-0468,1573-7330
DOI: 10.1007/bf02212934